Anti-SPS | Sucrose phosphate synthase, global

(Cat#: AS03 035A)
AS03_035A
AS03_035A_1
Description
  • Immunogen: KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution add 25 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000-1 : 5000 (WB)
  • Expected | apparent MW: 120 | 120-130 kDa (fragments of 30-90 kDa may be detected)
  • Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Miscanthus x giganteus, Pinus strobus, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
  • Bilska-Kos et al. (2020). Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus× giganteus and Zea mays at low temperature. Planta . 2020 Jul 16;252(2):23. doi: 10.1007/s00425-020-03421-2. Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.
  • Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants

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