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{{/_source.additionalInfo}}Anti-PsbA | D1 protein of PSII, C-terminal, Biotin-conjugated (40 µg)
(Cat#: AS05 084B)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
- Format: Liquid
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 15 000 (WB)
- Expected | apparent MW: 38 | 28-30 kDa
- Confirmed reactivity: Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma sp., Coscinodiscus wailesii, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana,Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Physcomitrium patens, Pinus strobus, rochlorococcus sp. (surface and deep water ecotype), Synechococcus sp. PCC 7942, Spirodela polyrhiza, Symbiodinium sp., Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.
- The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the chicken anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.
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