Anti-FeSOD | Chloroplastic Fe-dependent superoxide dismutase

(Cat#: AS06 125)
AS06_125
AS06_125_1
Description
  • Immunogen: overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1500-1 : 5000 (WB)
  • Expected | apparent MW: 25 | 22 kDa
  • Confirmed reactivity: Armeria maritima, Arabidopsis thaliana, Brassica juncea, Chlamydomonas reinhardtii, Dunaliella bardawil, Dunaliella salina, Fremyella diplosiphon, Medicago sativa, Morus spp., Oryza sativa, Salicornia sp., Solanum tuberosum, winter triticale, Zea mays
  • Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
  • Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Konkolewska et al. (2020). Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd). Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0.
  • The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251Out of three FeSOD isoforms, FeSOD2 and FeSOD3 are not expressed in the roots. In roots of Arabidopsis thaliana, FeSOD1 is detected Takáč et al. (2018)This product can be sold containing ProClin if requested

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