Anti-PsbD | D2 protein of PSII

(Cat#: AS06 146)
AS06_146
AS06_146_1
Description
  • Immunogen: KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis thaliana P56761, Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Clear-native PAGE (CN-PAGE), Western blot (WB)
  • Recommended dilutions: 1: 10 000 (CN-PAGE), 1: 5000 - 1 : 50 000 (WB)
  • Expected | apparent MW: 39.4 | 28-30 kDa
  • Confirmed reactivity: Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Chromochloris zofingiensis, Echinola crus-galli, Emiliania huxleyi, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Skeletonema costatum (diatom), Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Triticale, Ulva prolifera, Zea mays
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39.5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.
  • Zhao et al. (2024). Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844. Ciesielska et al. (2024). S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318. Khaig and Eaton-Rye (2023). Lys264 of the D2 Protein Performs a Dual Role in Photosystem II Modifying Assembly and Electron Transfer through the Quinone–Iron Acceptor Complex. Biochemistry 2023, 62, 18, 2738–2750 Jiang et al. (2023). Toxic effects of lanthanum (III) on photosynthetic performance of rice seedlings: Combined chlorophyll fluorescence, chloroplast structure and thylakoid membrane protein assessment. Ecotoxicol Environ Saf. 2023 Nov 15:267:115627.doi: 10.1016/j.ecoenv.2023.115627. Rredhi et al. (2023). The UV-A Receptor CRY-DASH1 Up- and Downregulates Proteins Involved in Different Plastidial Pathways. J Mol Biol. 2023 Sep 10:168271.doi: 10.1016/j.jmb.2023.168271.Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.von Bismarck et al. (2021) Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis. Research Square; 2021. DOI: 10.21203/rs.3.rs-948381/v1.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.
  • There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

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