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{{/_source.additionalInfo}}Anti-ATG8 | Autophagy-related protein
(Cat#: AS14 2769)
Description
- Immunogen: Fragment of recombinant ATG8 from Chlamydomonas reinhardtii, UniProt: A8JB85
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunolocalization (IL), Western blot (WB)
- Recommended dilutions: 1 : 1000 (IL), 1 : 1000-1 : 2000 (WB)
- Expected | apparent MW: 15.2 | 15 kDa
- Confirmed reactivity: Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Chlorococcum dorsiventrale, Haematococcus lacustris, Nicotiana benthamiana, Populus trichocarpa, Solanum lycopersicum, Zea mays
- Not reactive in: Cuscuta chinensis, Symbiodinium sp.
- ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al. 2010, Plant Physiol. 152: 1874-88).
- Li et al. (2024). Gamma-aminobutyric acid as a regulator of astaxanthin production in Haematococcus lacustris under salinity: Exploring physiology, signaling, autophagy, and multi-omics landscape. Bioresour Technol. 2024 Dec:413:131466.doi: 10.1016/j.biortech.2024.131466. Epub 2024 Sep 12. Michalak et al. (2024). Conserved autophagy and diverse cell wall composition: Unifying features of vascular tissues in evolutionarily distinct plants.Ann Bot. 2024 Apr 23;133(4):559-572. doi: 10.1093/aob/mcae015. Lan et al. (2024).Clathrin Light Chains negatively regulate plant immunity by hijacking the autophagy pathway. Plant Commun. 2024 Apr 30:100937.doi: 10.1016/j.xplc.2024.100937. Testi et al. (2024). The Phytophthora parasitica effector AVH195 interacts with ATG8, attenuates host autophagy, and promotes biotrophic infection. BMC Biol. 2024 Apr 29;22(1):100.Michalak et al. (2024). Conserved autophagy and diverse cell wall composition: Unifying features of vascular tissues in evolutionarily distinct plants. Ann Bot. 2024 Feb 7:mcae015. (immunofluorescence) doi: 10.1093/aob/mcae015. Llamas et al. (2023). In planta expression of human polyQ-expanded huntingtin fragment reveals mechanisms to prevent disease-related protein aggregation. Nat Aging. 2023 Nov;3(11):1345-1357.doi: 10.1038/s43587-023-00502-1. Miklaszewska et al. (2023).CALEOSIN 1 interaction with AUTOPHAGY-RELATED PROTEIN 8 facilitates lipid droplet microautophagy in seedlings.Plant Physiol. 2023 Nov 22;193(4):2361-2380.doi: 10.1093/plphys/kiad471. Lee et al. (2023).Three consecutive cytosolic glycolysis enzymes modulate autophagic flux. Plant Physiol. 2023 Oct 26;193(3):1797-1815. doi: 10.1093/plphys/kiad439.Sun et al. (2022) Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass. Nat Commun. 2022;13(1):7731. Published 2022 Dec 13. doi:10.1038/s41467-022-35507-8Cao et al. (2022) Autophagic pathway contributes to low-nitrogen tolerance by optimizing nitrogen uptake and utilization in tomato. Hortic Res. 2022 Mar 23;9:uhac068. doi: 10.1093/hr/uhac068. PMID: 35669705; PMCID: PMC9164271.Samperna et al (2022). Cyclopaldic Acid, the Main Phytotoxic Metabolite of Diplodia cupressi, Induces Programmed Cell Death and Autophagy in Arabidopsis thaliana. Toxins (Basel). 2022 Jul 11;14(7):474. doi: 10.3390/toxins14070474. PMID: 35878212; PMCID: PMC9325063.
- For Arabidopsis thaliana the signal obtained using ATG8 antibodies is cleaner in case of roots compare to leaf material. For best results please follow extraction protocol described in Álvarez et al. (2012). ATG8 signal corresponds to the two bands of 17 kDa.Preparation of a cell extract from Arabidopsis thaliana:A. Plants were first subjected to autophagy activating conditions: nutrient (nitrogen or carbon) limitation or oxidative stress in order to activate this degradative process.B. Total protein extracts can be obtained as described by Álvarez. Leaves are grinded in liquid nitrogen with a minimal volume of extraction buffer (100 mM Tris-HCl pH 8, 400 mM sucrose, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml sodium deoxycholate, 10 µg/ml of leupeptin, 10 µg/ml of pepstatin A, 4% (v/v) protease inhibitor cocktail from Roche).C. Cell debris is removed by centrifuging at 500 g for 10 min at 4°C.Important note:It is recommendable to use bigger gels in order to get a better resolution of ATG8 bands. Midi-protean gels are better than mini-gels. There are 9 ATG8 isoforms and this antibody will likely recognizes all of them.For immunolocalization protocol, please inquire.
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