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{{/_source.additionalInfo}}Anti-Cat | Catalase (peroxisomal marker)
(Cat#: AS09 501)
Description
- Immunogen: KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunogold (IG), Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1: 500 (IG), 1: 25 - 1: 500 (IL), 2 µg (IP), 1 : 1000 (WB)
- Expected | apparent MW: 57 | 55 kDa
- Confirmed reactivity: Fragaria x ananassa, Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
- Not reactive in: Chlamydomonas reinhardtii
- Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
- Niemiro et al. (2024). LSU family members and NBR1 are novel factors that contribute to homeostasis of catalases and peroxisomes in Arabidopsis thaliana. Sci Rep. 2024 Oct 25;14(1):25412. doi: 10.1038/s41598-024-76862-4. Hu et al. (2023).Catalase associated with antagonistic changes of abscisic acid and gibberellin response, biosynthesis and catabolism is involved in eugenol-inhibited seed. Plant Cell Rep. 2023 Dec 23;43(1):10.doi: 10.1007/s00299-023-03096-5. Melicher et al. (2023). Methyl viologen-induced changes in the Arabidopsis proteome implicate PATELLIN 4 in oxidative stress responses.J Exp Bot. 2024 Jan 1;75(1):405-421.doi: 10.1093/jxb/erad363. Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Wilmowicz et al (2021) EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. International Journal of Molecular Sciences. 2021; 22(6):3001. https://doi.org/10.3390/ijms22063001Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Adamiec et al. (2021). Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease. PHOTOSYNTHETICA 59 (4): 633-639, 2021, DOI 10.32615/ps.2021.053
- To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.
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