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{{/_source.additionalInfo}}Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)
(Cat#: AS07 212B)
Description
- Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5 , TAIR: AT3G01280, VDAC2 UniProt F4K3R8-1 , TAIR: AT5G67500, VDAC3 UniProt: Q9SMX3-1, TAIR: AT5G15090, VDAC4 UniProt: Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt: Q9M2W6-1, TAIR: AT3G49920
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
- Format: Liquid
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- Tested applications: 2D Blue Native PAGE (2D BN-PAGE), Immunolocalization (IL), Western blot (WB)
- Recommended dilutions: 1 : 500 (IL), 1 : 2000, 2-30 µg protein/lane (WB)
- Expected | apparent MW: 29 kDa (for Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens
- Not reactive in: Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae
- VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
- Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
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