Anti-SAL1 | Sal1 phosphatase

(Cat#: AS07 256)
AS07_256
AS07_256_1
Description
  • Immunogen: Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (At5g63980, protein Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 100 µl
  • Reconstitution: For reconstitution add 100 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Expected | apparent MW: 37.5 | 41 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Glycine max, Lycopersicum esculentum, Nicotiana tabaccum, Populus tremula
  • Not reactive in: Chlamydomonas reinhardii
  • The plant SAL1 (known also as FIERY1, FRY1, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2, HOS2, MBM17.8, MBM17_8) is a 353 aa protein homologous to the HAL2 and CysQ phosphatases of yeast and Escherichia coli, respectively. The SAL1 protein expressed in E. coli shows nucleotidase and inositol phosphatase activities. SAL1 is proposed to participate in the sulfur assimilation pathway as well as in the phosphoinositide signaling pathway.
  • Chan et al. (2016). Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):E4567-76. doi: 10.1073/pnas.1604936113. Epub 2016 Jul 18.

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