Anti-Pht1-1/2 | Root phosphate transporter isoform 1-1 and 1-2

(Cat#: AS08 321)
Description
  • Immunogen: two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 100 µl
  • Reconstitution: For reconstitution add 100 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
  • Recommended dilutions: 1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
  • Expected | apparent MW: 56.9 kDa
  • Confirmed reactivity: Hordeum vulgare
  • Not reactive in: Arabidopsis thaliana
  • Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).
  • Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
  • Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation

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