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{{/_source.additionalInfo}}Anti-AGO1 | Argonaute 1
(Cat#: AS09 527)
Description
- Immunogen: KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunolocalization (IL), small-RNA-IP-Seq, Western blot (WB)
- Recommended dilutions: 2 µg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)
- Expected | apparent MW: 116.4 | 130 kDa
- Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
- Not reactive in: Chlamydomonas reinhardtii, Medicago sativa, Salvia sp., Spinacia oleracea, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea maysMammals
- AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
- Li et al. (2024). RNA helicase Brr2a promotes miRNA biogenesis by properly remodelling secondary structure of pri-miRNAs. Nat Plants . 2024 Oct;10(10):1532-1547. doi: 10.1038/s41477-024-01788-8. Mirlohi et al. (2024). An AGO10: miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root. EMBO J. 2024 May;43(9):1843-1869.doi: 10.1038/s44318-024-00071-y. Martín-Merchán et al. (2024). Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387. Barre-Villeneuve et al. (2024). The unique dual targeting of AGO1 by two types of PRMT enzymes promotes phasiRNA loading in Arabidopsis thaliana. Nucleic Acids Res. 2024 Mar 21;52(5):2480-2497. doi: 10.1093/nar/gkae045.Blagojevic et al. (2024). Heat stress promotes Arabidopsis AGO1 phase separation and association with stress granule components. iScience. 2024 Feb 6;27(3):109151. doi: 10.1016/j.isci.2024.109151. eCollection 2024 Mar 15. Bradamante et al. (2023). Two ARG ONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis. Plant Cell. 2023 Dec 7:koad295.doi: 10.1093/plcell/koad295. Meng et al. (2022) The novel activity of Argonautes in intron splicing: A transcriptome-wide survey in plants. J Plant Physiol. 2022 Jan 31;270:153632. doi: 10.1016/j.jplph.2022.153632. Epub ahead of print. PMID: 35114616.Cabezas-Fuster et al. (2022). Missplicing suppressor alleles of Arabidopsis PRE-MRNA PROCESSING FACTOR 8 increase splicing fidelity by reducing the use of novel splice sites. Nucleic Acids Res. 2022 Jun 10;50(10):5513-5527. doi: 10.1093/nar/gkac338. PMID: 35639749; PMCID: PMC9177961.Delenko et al. (2022) MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal. BMC Plant Biol. 2022 Jan 3;22(1):9. doi: 10.1186/s12870-021-03323-9. PMID: 34979922; PMCID: PMC8722089. (immunofluorescence)Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Dalmadi et al. (2021) Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis. Nucleic Acids Res. 2021 Dec 16;49(22):12912-12928. doi: 10.1093/nar/gkab1138. PMID: 34850097; PMCID: PMC8682782.
- AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method
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