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{{/_source.additionalInfo}}Anti-AGO1 | Argonaute 1 (40 µg, ALP-conjugated)
(Cat#: AS09 527-ALP)
Description
- Immunogen: KLH conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 UniProt:O04379, TAIR: At1g48410.
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
- Format: Liquid
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 116.4 | 130 kDa
- Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
- Not reactive in: Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays
- AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Alkaline Phosphatase (ALP).
- AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method
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