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{{/_source.additionalInfo}}Anti-AGO1 | Argonaute 1, DyLight® 594 conjugated (40 µg)
(Cat#: AS09 527-DL594)
Description
- Immunogen: KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
- Format: Liquid in PBS pH 7.4.
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: To be determined by end user.
- Expected | apparent MW: 116.4 | 130 kDa
- Confirmed reactivity: Arabidopsis thaliana, Nicotiana benthamiana
- Not reactive in: Chlamydomonas reinhardtii
- AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). This antibody is directly conjugated to Horseradish Peroxidase (HRP).
- To be added when available. Antibody released in May 2023.
- AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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