Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

(Cat#: AS09 580)
AS09_580
AS09_580_1
AS09_580_1
Description
  • Immunogen: KLH-conjugated peptide derived from known aadA1 protein sequences
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 3 000 (WB)
  • Expected | apparent MW: 30 | 30 kDa
  • Confirmed reactivity: aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
  • Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.

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