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{{/_source.additionalInfo}}Goat anti-Rat IgG (H&L), HRP conjugated
(Cat#: AS10 1187)
Description
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified rat IgG.
- Format: Lyophilized
- Quantity: 1 mg
- Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Store liquid material at 2-8°C up to 6 months.
- Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: The optimal working dilution should be determined by the investigator
- Confirmed reactivity: Heavy chains on rat IgG and with the light chains on all rat immunoglobulins
- Not reactive in: Non-immunoglobulin rat serum proteins
- Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
- Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.
- Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.
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