Goat anti-biotin, HRP conjugated

(Cat#: AS10 1454)
Description
  • Immunogen: Biotin
  • Host: Goat
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified goat IgG.
  • Format: Lyophilized
  • Quantity: 1 mg
  • Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
  • Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
  • Recommended dilutions: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
  • Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
  • Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth

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