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{{/_source.additionalInfo}}Rabbit anti-Chicken IgY (H&L), HRP conjugated
(Cat#: AS10 1489)
Description
- Immunogen: Purified Chicken IgY (H&L), whole molecule
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified rabbit IgG.
- Format: Lyophilized
- Quantity: 1 mg
- Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
- Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: The optimal working dilution should be determined by the investigator
- Confirmed reactivity: Heavy chains on chicken IgY and light chains on all chicken immunoglobulins,
- Not reactive in: Non-immunoglobulin chicken serum proteins
- Rabbit anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgY.Chicken egg yolk immunoglobulin (IgY) has been also called chicken IgG.
- Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151Panayiotou et al. (2018). Viperin restricts Zika virus and tick-borne encephalitis virus replication by targeting NS3 for proteasomal degradation.J Virol. 2018 Jan 10. pii: JVI.02054-17. doi: 10.1128/JVI.02054-17.
- Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
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