Rabbit anti-Human IgG (H&L), FITC conjugated

(Cat#: AS10 1502)
Description
  • Immunogen: Purified human IgG (H&L) CAA10835
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified rabbit IgG.
  • Format: Liquid
  • Quantity: 2 mg
  • Storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
  • Tested applications: FELISA (Fluorescent ELISA), FACS (Fluorescence-activated cell sorting), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Recommended dilutions: 1: 20 -1: 20 000 (IHC)
  • Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to FITC, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
  • Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.

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