Anti-Enolase 2

(Cat#: AS10 651)
AS10_651
AS10_651_1
Description
  • Immunogen: Recombinant Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 200 µl
  • Reconstitution: For reconstitution add 200 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 2000 (WB)
  • Expected | apparent MW: 47.7 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Helianthus annuus
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator
  • Zhang et al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w. Zhang et al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Chen et al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.
  • Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below

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