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{{/_source.additionalInfo}}Anti-AGO5 | Argonaute 5
(Cat#: AS10 671)
Description
- Immunogen: peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
- Expected | apparent MW: 111 | 111 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Hordeum vulgare, Solanum lycopersicum, Zea mays
- AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
- Martín-Merchán et al. (2024). Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387. Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.
- AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure
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