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{{/_source.additionalInfo}}Anti-AGO6 | Argonaute 6
(Cat#: AS10 672)
Description
- Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 116.4 | 99 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Zea mays
- AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
- Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956 Havecker el al. (2010). The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199.
- AGO6 expression is cell-specific. Seedlings can be used as a negative control
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