Anti-ADH | Alcohol dehydrogenase (hypoxia marker)

(Cat#: AS10 685)
AS10_685
AS10_685_1
Description
  • Immunogen: KLH-conjugated peptide derived from available ADH sequences including Arabidopsis thaliana P06525, At1g77120
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water.
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 3000 (WB)
  • Expected | apparent MW: 42 | 42 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Fragaria vesca, Oryza sativa, Phaseolus vulgaris, Solanum lycopersicum
  • Not reactive in: Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii.
  • Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress. It is known to be synthesized preferentially under low oxygen conditions.
  • Zhang et al. (2024). BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition. Plant Cell. 2024 Apr 12:koae117. doi: 10.1093/plcell/koae117. Czernicka et al. (2022). Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming. Cells. 2022 Jan 31;11(3):500. doi: 10.3390/cells11030500. PMID: 35159309; PMCID: PMC8834170.Ventura et al. (2020). Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth. Sci Rep. 2020 Oct 7;10(1):16669. doi: 10.1038/s41598-020-73704-x. PMID: 33028901; PMCID: PMC7542448.Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355.Bui et al. (2019). Conservation of ethanol fermentation and its regulation in land plants. J Exp Bot. 2019 Feb 28. pii: erz052. doi: 10.1093/jxb/erz052.De la Rosa et al. (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit. Plant Cell Environ. 2019 Jan;42(1):133-144. doi: 10.1111/pce.13209.

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