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{{/_source.additionalInfo}}Anti-H3 | Histone H3 (antigen affinity purified)
(Cat#: AS10 710A)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ChIp-qPCR (ChIp-qPCR), Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 2,5 µg/100 µg of chromatin (ChIp-qPCR), 1: 400 (IF), 1 : 5000 (WB)
- Expected | apparent MW: 15 | 17 kDa
- Confirmed reactivity: Arabidopsis thaliana, Oryza sativa
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
- Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific protol for ChIP can be found here: Saleh et al. (2008).
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