Goat anti-Human IgE heavy (epsilon chain), ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

(Cat#: AS10 756)
Description
  • Immunogen: Purified human IgE
  • Host: Goat
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified goat IgG.
  • Format: Liquid
  • Quantity: 0.5 mg
  • Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
  • Recommended dilutions: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Confirmed reactivity: Human IgE heavy (epsilon chain)
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.
  • ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA. Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

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