Donkey anti-Rabbit IgG (H&L), HRP conjugated

(Cat#: AS10 845)
AS10_845
AS10_845
AS10_845
Description
  • Immunogen: Purified Rabbit IgG
  • Host: Donkey
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified donkey IgG.
  • Format: Lyophilized
  • Quantity: 1 mg
  • Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
  • Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
  • Recommended dilutions: The optimal working dilution should be determined by the investigator
  • Confirmed reactivity: Rabbit IgG (H&L)
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
  • Cook et al. (2024). Chloroplast phosphatases LPPγ and LPPε1 facilitate conversion of extraplastidic phospholipids to galactolipids. Plant Physiol. 2024 May 31;195(2):1506-1520. doi: 10.1093/plphys/kiae100.
  • HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

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