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{{/_source.additionalInfo}}Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB)
(Cat#: AS10 932)
Description
- Immunogen: Partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V. The epitope is 3-8. The molecular weight of immunogen is 4.5 kDa.
- Host: Mouse
- Clonality: Monoclonal
- Purity: Affinity purified in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Recommended dilutions: Coating antibody at 2 µg/ml (ELISA), 1: 1000 (IF), 1 : 500 (IHC)
- Expected | apparent MW: 4.5 kDa
- Confirmed reactivity: Human Abeta oligomers only
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
- Nagashima et al. (2024). Development of Triphenylmethane Dyes for In Vivo Fluorescence Imaging of Aβ Oligomers. ACS Chem Neurosci. 2024 Jun 5;15(11):2233-2242. doi: 10.1021/acschemneuro.4c00053. Akasanka et al. (2023). In Vivo Near-Infrared Fluorescence Imaging Selective for Soluble Amyloid β Aggregates Using y-Shaped BODIPY Derivative.J Med Chem. 2023 Oct 26;66(20):14029-14046.doi: 10.1021/acs.jmedchem.3c01057. Pang et al (2021) An App knock-in rat model for Alzheimer's disease exhibiting A? and tau pathologies, neuronal death and cognitive impairments. Cell Res. 2021 Nov 17. doi: 10.1038/s41422-021-00582-x. Epub ahead of print. PMID: 34789895.Oh et al. (2020). Associative Interactions among Zinc, Apolipoprotein E, and Amyloid-? in the Amyloid Pathology. Int J Mol Sci. 2020 Jan 25;21(3). pii: E802. doi: 10.3390/ijms21030802.Henning-Knechtel et al. (2020). Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity. Cell Reports Physical Science,Volume 1, Issue 2, 26Zhang et al. (2019). Brains of rhesus monkeys display A? deposits and glial pathology while lacking A? dimers and other Alzheimer's pathologies. Aging Cell. 2019 Jun 4:e12978. doi: 10.1111/acel.12978.Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of A? Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401.
- OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
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