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{{/_source.additionalInfo}}Anti-eEF1a | Elongation factor 1-alpha
(Cat#: AS10 934)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from known sequences of eEF1a including Arabidopsis thaliana EF-1-alpha 1P0DH99 AT1G07940, EF-1-alpha 2 Q8W4H7 AT1G07930, EF-1-alpha 3 Q0WL5 AT1G07920, EF-1-alpha 4 Q8GTY0 AT5G6039
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 49.5 kDa
- Confirmed reactivity: Arabidopsis thaliana, Avena sativa, Fraxinus pennsylvanica, Hordeum vulgare, Olea europaea, Oryza sativa, Physcomitrium patens, Solanum tuberosum, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
- Yan et al. (2024). SUBSTANDARD STARCH GRAIN7 regulates starch grain size and endosperm development in rice. Plant Biotechnol J. 2024 Aug 24. doi: 10.1111/pbi.14444. Yan et al. (2023). OsSHMT4 Is Required for Synthesis of Rice Storage Protein and Storage Organelle Formation in Endosperm Cells. Plants 2024, 13(1), 81; https://doi.org/10.3390/plants13010081,Pan et al. (2021) Post-Golgi Trafficking of Rice Storage Proteins Requires the Small GTPase Rab7 Activation Complex MON1-CCZ1, Plant Physiology, 2021;, kiab175, https://doi.org/10.1093/plphys/kiab175Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.Djukic et al. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. Journal of Plant Physiology Volume 240, September 2019, 153015.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
- This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.