Goat anti-Human IgG Fc, FITC conjugated

(Cat#: AS10 977)
Description
  • Host: Goat
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified goat IgG.
  • Format: Liquid
  • Quantity: 2 mg
  • Storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt.
  • Recommended dilutions: 1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)
  • Goat anti-human IgG Fc is a FITC conjugated secondary antibody which binds to human IgG upsilon in immunological assays. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. The ratio is 8.3 Moles FITC per Mole Antibody. Antibodies are affinity purified using solid phase human IgG.
  • This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins. Minimum cross-reactivity is observed to human IgG, F(ab)'2 fragment based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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