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{{/_source.additionalInfo}}Anti-DMPO | DMPO nitrone adduct (clone N1664A)
(Cat#: AS11 1632)
Description
- Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
- Host: Mouse
- Clonality: Monoclonal
- Purity: Total IgG. Protein G purified.
- Format: Liquid
- Quantity: 100 µl
- Storage: Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)
- Expected | apparent MW: 90 kDa
- Confirmed reactivity: DMPO (species independent)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
- Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
- Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct
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