Anti-His-tag | 6xHis (clone HIS,H8 / EH158)

(Cat#: AS11 1771)
AS11_1771
AS11_1771
Description
  • Immunogen: KLH-conjugated synthetic peptide 6xHis
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: Total IgG fraction. Protein A purified.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store at -20°C.
  • Tested applications: Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunlocalization (IL), Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Confirmed reactivity: 6xHis
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
  • De Brasi-Velasco et al. (2021). Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions. Antioxidants. 2021; 10(12):1884. https://doi.org/10.3390/antiox10121884Tan et al. (2020). Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants. Curr Biol. 2020 Feb 3;30(3):381-395.e8. doi: 10.1016/j.cub.2019.11.058.López-Vidal et al. (2020). Is Autophagy Involved in Pepper Fruit Ripening? Cells, 9 (1) , DOI: 10.3390/cells9010106 Häggmark-Månberg et al. (2016). Autoantibody targets in vaccine-associated narcolepsy. Autoimmunity. 2016 Sep;49(6):421-433. Epub 2016 May 20.
  • Working dilution for ELISA, IL and IP needs to be determined experimentally

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