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{{/_source.additionalInfo}}Goat anti-Mouse IgG (H&L), HRP conjugated
(Cat#: AS11 1772)
Description
- Immunogen: Purified mouse IgG (H&L), whole molecule, AAA51107
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified goat IgG.
- Format: Lyophilized
- Quantity: 1 mg
- Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
- Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: The optimal working dilution should be determined by the investigator
- Confirmed reactivity: Heavy chains on mouse IgG and light chains on all mouse immunoglobulins
- Not reactive in: Non-immunoglobulin mouse serum proteins
- Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L).
- Chung et al. (2024). Identification and characterization of the COPII vesicle-forming GTPase Sar1 in Chlamydomonas. Plant Direct. 2024 Jun 16;8(6):e614. doi: 10.1002/pld3.614. Mets et al. (2024). Mechanism of phage sensing and restriction by toxin-antitoxin-chaperone systems. Cell Host Microbe . 2024 May 23:S1931-3128(24)00174-4. doi: 10.1016/j.chom.2024.05.003. Hemmel et al. (2024). Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant Mol Biol. 2024 Feb 14;114(1):18.doi: 10.1007/s11103-024-01415-2. Chung et al. (2023). An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression. Nucleic Acids Res. 2023 Nov 10;51(20):11386-11400. doi: 10.1093/nar/gkad816. Kasari et al. (2019). A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling. Nucleic Acids Res. 2019 Jul 12. pii: gkz600. doi: 10.1093/nar/gkz600.Li and Bock (2018). Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum. Nat Commun. 2018 Aug 27;9(1):3451. doi: 10.1038/s41467-018-05651-1.Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
- Concentration: 1.0 mg/ml. Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) ProClin 150 as preservative of bacterial growth.The antibody will detect all isotypes of mouse IgG.
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