Anti-ACD1 | Accelerated cell death 1

(Cat#: AS11 1783)
AS11_1783
AS11_1783_1
Description
  • Immunogen: Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 5000 (WB)
  • Expected | apparent MW: 61 | 54 kDa
  • Confirmed reactivity: Arabidopsis thaliana
  • Not reactive in: Pinus strobus
  • Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase
  • Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Kim et al. (2013). Mutation of the Arabidopsis NAC016 Transcription Factor Delays Leaf Senescence.'Plant Cell Physiol. Aug 21.Nagane et al. (2010). Involvement of AtNAP1 in thre reulation of chlorophyll degradation in Arabiopsis thaliana. Planta (4):939-949.Hirashima et al. (2009). Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana. Plant Cell Physiol. (4):719-729.
  • This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.

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