Anti-5MeC | 5-Methylcytosine (clone 5MC-CD) biotinylated

(Cat#: AS21 4560)
AS21_4560
AS21_4560_1
Description
  • Immunogen: BSA-conjugated 5-Methylcytosine
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: Purified IgM in PBS. Contains 50 % glycerol, filter sterilized, biotinylated.
  • Format: Liquid
  • Quantity: 50 µg at 1 µg/µl
  • Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Confirmed reactivity: Chlamydmonas me-1 cells, mouse embryonic stem cells
  • DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells.

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