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{{/_source.additionalInfo}}Goat anti-Rabbit IgG (H&L), DyLight® 350 conjugated
(Cat#: AS11 1812)
Description
- Immunogen: Purified Rabbit IgG, whole molecule
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified goat IgG.
- Format: Lyophilized
- Quantity: 1 mg
- Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
- Tested applications: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Recommended dilutions: 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
- Confirmed reactivity: Rabbit IgG heavy and light chains (H&L)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Goat anti-rabbit IgG, DyLight® 350 Conjugate is a secondary antibody conjugated toDyLight® 350, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
- Conjugate is present in 10 mM Sodium Phosphate,0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 350 has a maximum absorbance at 353 nm; Emax = 432 nm.
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