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{{/_source.additionalInfo}}Anti-HY5 | Protein long hypocotyl 5
(Cat#: AS12 1867)
Description
- Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HY5 protein sequence.
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1: 500 to 1 : 1000 (WB)
- Expected | apparent MW: 18.5 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus sp., Solanum lycopersicum, Triticum aestivum, Zea mays
- HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.
- Yao et. al. 2024).Cooperative transcriptional regulation by ATAF1 and HY5 promotes light-induced cotyledon opening in Arabidopsis thaliana. Sci Signal. 2024 Jan 2;17(817):eadf7318. Liu et al. (2024). Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation. Nat Commun. 2024 Feb 9;15(1):1221.doi: 10.1038/s41467-024-45575-7.Cazzonelli et al. (2019). A cis-carotene derived apocarotenoid regulates etioplast and chloroplast development. https://doi.org/10.1101/528331Lee et al. (2017). The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering. Nat Commun. 2017 Dec 22;8(1):2259. doi: 10.1038/s41467-017-02476-2.Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.
- This antibody detects recombinant HY5 in Nicotiana benthamiana.Extraction and loading buffer with 6-8 M urea buffer needs to be used when working with endogenous extract to allow detection with this antibody or TCA/acetone protein extraction or as described in Mechin et al. (2007).Samples need to be harvested under dim-green safe light conditions, to avoid degradation during harvesting and extraction process.
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