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{{/_source.additionalInfo}}Chicken anti-Goat IgG (H&L), DyLight® 633 conjugated, min, cross-reactivity to human, mouse or rabbit IgG and serum proteins
(Cat#: As12 2130)
Description
- Immunogen: Purified goat IgG, whole molecule
- Host: Chicken
- Clonality: Polyclonal
- Purity: Immunogen affinity purified chicken IgG.
- Format: Lyophilized
- Quantity: 0.5 mg
- Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
- Recommended dilutions: 1 : 20-1 : 2000 for most applications
- Chicken anti-Goat IgG (H&L) - DyLight® 633 Conjugated, min. cross-reactivity to human, mouse or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 633, which binds to goat IgG (H&L) in immunological assays. DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
- Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA. Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative
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