Anti-L13-1 | 60S ribosomal protein L13-1

(Cat#: AS13 2650)
AS13_2650
AS13_2650
Description
  • Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P41127, TAIR: At3g49010
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Antigen affinity purified serum in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution add 50 µl of steril water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 2500 (WB)
  • Expected | apparent MW: 23.7 | 29 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamiana, Zea mays
  • Not reactive in: Diatoms
  • L13 protein is localized in the cytoplasm. It belongs to the ribosomal protein L13e family. Alternative name: protein BBC1 homolog.
  • Wang et al. (2024). Maize Dek51 encodes a DEAD-box RNA helicase essential for pre-rRNA processing and seed development. Cell Rep. 2024 Sep 24;43(9):114673. doi: 10.1016/j.celrep.2024.114673. Lee et al. (2023). The Phytophthora nucleolar effector Pi23226 targets host ribosome biogenesis to induce necrotrophic cell death. Plant Commun. 100606. doi: 10.1016/j.xplc.2023.100606. Pereira Firmino et al. (2020). Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes. Plants (Basel) . 2020 Jul 14;9(7):892.doi: 10.3390/plants9070892. Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.Li (2019). The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs. Methods Mol Biol. 2019;1932:317-333. doi: 10.1007/978-1-4939-9042-9_23.You et al. (2019). FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis. Nat Commun. 2019 Sep 27;10(1):4424. doi: 10.1038/s41467-019-12379-z.de Francisco Amorim et al. (2018). The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing. Plant Cell. 2018 Nov;30(11):2838-2854. doi: 10.1105/tpc.18.00244. Epub 2018 Oct 11.Beine-Golovchuk et al. (2018). Plant Temperature Acclimation and Growth Rely on Cytosolic Ribosome Biogenesis Factor Homologs. Plant Physiol. 2018 Mar;176(3):2251-2276. doi: 10.1104/pp.17.01448. Epub 2018 Jan 30.Wang et al. (2016). Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis. J Exp Bot. 2016 Dec;67(22):6323-6335. Epub 2016 Oct 27.
  • Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

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