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{{/_source.additionalInfo}}Anti-CCA1 | Circadian clock associated 1
(Cat#: AS13 2659)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Co-immunoprecipitation (Co-IP), Western blot (WB)
- Recommended dilutions: 1: 500 (Co-IP), 1 : 500 (WB)
- Expected | apparent MW: 67 | 80 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Oryza sativa, Solanum lycopersicum
- CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
- Hung er al. (2018). The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes. Nucleic Acids Res. 2018 Aug 14. doi: 10.1093/nar/gky749.
- Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100°C heating block, not at RT. Centrifugation for 5 minutes will remove cell debris. Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots. Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
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