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{{/_source.additionalInfo}}Anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated
(Cat#: AS13 2664)
Description
- Immunogen: KLH-conjugated peptide containing phospho-Thr, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Never Store this antibody in 4°C
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 500 (WB)
- Expected | apparent MW: 52.6 kDa (S6K1) and 53 kDa (S6K2) | 60 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).
- Tanigawa et al. (2024). FYVE1/FREE1 is involved in glutamine-responsive TORC1 activation in plants. iScience. 2024 Aug 26;27(9):110814. doi: 10.1016/j.isci.2024.110814. Li et al. (2023). Mitigating growth-stress tradeoffs via elevated TOR signaling in rice. Mol Plant. 2023 Dec 5:S1674-2052(23)00396-9. doi: 10.1016/j.molp.2023.12.002. González-López et al. (2021). Growth promotion in Arabidopsis thaliana by bacterial cyclodipeptides involves the TOR/S6K pathway activation. Journal of Plant Physiology. Volume 257, 2021, 153343,ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2020.153343.Kazibwe et al. (2020). TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant. J Exp Bot. 2020 Sep 9;eraa410.doi: 10.1093/jxb/eraa410.Dealy et al. (2019). CEP3 levels affect starvation-related growth responses of the primary root. J Exp Bot. 2019 Jun 6. pii: erz270. doi: 10.1093/jxb/erz270.Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Wang et al. (2017). Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response. Mol Cell. 2017 Dec 27. pii: S1097-2765(17)30930-9. doi: 10.1016/j.molcel.2017.12.002.
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