Anti-H+ATPase | Plasma membrane H+ATPase (chicken antibody)

(Cat#: AS13 2671)
AS13_2671
AS13_2671_1
Description
  • Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
  • Host: Chicken
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified IgY in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000-1 : 5000 (WB)
  • Expected | apparent MW: 95 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Spinacia oleracea, Zea mays
  • The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
  • VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot

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