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{{/_source.additionalInfo}}Anti-MKKK18 | Mitogen-activated protein kinase 18
(Cat#: AS13 2673)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana kinase 18 sequence UniProt: Q9ZVP5, TAIR: AT1G05100
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 3 µl (IP), 1 : 1000 (WB)
- Expected | apparent MW: 37.7 | 38 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Mitogen-activated protein kinase 18 has ATP binding and serine/threonine kinase activity. Alternative names: T7A14.2 protein, Putative NPK1-related MAP kinase.
- Tajdel-Zielińska et al. (2023). Arabidopsis HECT and RING-type E3 Ligase Promote MAPKKK18 Degradation To Regulate Abscisic Acid Signalling. Plant Cell Physiol. 2023 Dec 28:pcad165.doi: 10.1093/pcp/pcad165. Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
- MKKK18 is not stable in endogenous extracts and to allow succesfull detection use transgenic plants or transient expression in protoplasts
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