Anti-RH3 | RNA helicase (chloroplastic)

(Cat#: AS13 2714)
AS13_2714
AS13_2714_1
Description
  • Immunogen: Recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01) and Arabidopsis thaliana At5g26742
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Serum
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunoprecipitation (IP), Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Expected | apparent MW: 75 kDa
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.
  • Bach-Pages et al. (2020). Discovering the RNA-Binding Proteome of Plant Leaves With an Improved RNA Interactome Capture Method. Biomolecules. 2020 Apr 24;10(4):E661. doi: 10.3390/biom10040661.Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.

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