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{{/_source.additionalInfo}}Anti-Glyphosate
(Cat#: AS13 2739)
Description
- Immunogen: BSA-conjugated Glyphosate (coupled via EDAC/NHS) , Target: Glyphosate, CAS no,: 1071-83-6 from SIGMA,
- Host: Chicken
- Clonality: Polyclonal
- Purity: Total IgY. Purified by PEG precipitation.
- Format: Liquid
- Quantity: 1 ml (at 5 mg/ml)
- Storage: Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing. For long term storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
- Tested applications: ELISA (ELISA), Immunoaffinity chromatography (IAC)
- Recommended dilutions: 1 : 2000 (ELISA)
- Glyphosate (N-(phosphonomethyl)glycine) is a herbicide of a broad action spectrum which is used to kill broadleaf weeds and grasses known to compete with commercial crops world-wide. Due to its physico-chemical properties glyphosate easily enters the aqueous environment where it is rapidly converted into its main metabolite AMPA (aminomethylphosphonic acid).
- Majer-Baranyi et al. (2023). Application of Highly Sensitive Immunosensor Based on Optical Waveguide Light-Mode Spectroscopy (OWLS) Technique for the Detection of the Herbicide Active Ingredient Glyphosate. Biosensors (Basel). 2023 Jul 29;13(8):771. doi: 10.3390/bios13080771. Vestri et al. (2021). LSPR immuno-sensing based on iso-Y nanopillars for highly sensitive and specific imidacloprid detection. J Mater Chem B. 2021 Nov 17;9(44):9153-9161. doi: 10.1039/d1tb01344k. PMID: 34694310.Viirlaid et al. (2019). Immunoassay for rapid on-site detection of glyphosate herbicide. Environ Monit Assess. 2019 Jul 24;191(8):507. doi: 10.1007/s10661-019-7657-z.
- This antibody was used to prepare anti-glyphosate immunoaffinity column which showed to be very specific and retained glyphosate but not AMPA or glufosinate. Recover of glyphosate was ca. 100 %. The antibody is applicable for any type of (waste) water sample. The detection limit is 2,5 μg/l, while the end concentration is 100 μg/l. The standard curve runs from 5 – 100 μg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assay buffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.
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