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{{/_source.additionalInfo}}Anti-SAG12 | Senescence-specific cysteine protease SAG12
(Cat#: AS14 2771)
Description
- Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana SAG12 protein sequence, UniProt:Q38886, TAIR: AT5G45890
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 2000 (WB)
- Expected | apparent MW: 38 | 28 kDa
- Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare
- Not reactive in: Citrus sinensis
- Senescence-specific cysteine protease (SAG12) is a cysteine protease influenced by cytokinin, auxin and sugars and involved in developmental senescence specific cell death.
- Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B'? controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Frank et al. (2019). The Hordeum vulgare cysteine protease HvPAP14 plays a role in degradation of chloroplast proteins. J Exp Bot. 2019 Aug 12. pii: erz356. doi: 10.1093/jxb/erz356.
- Recommended plant extraction buffer: See Durian et al. (2019). Denature in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) at 70◦C for 5 min
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