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{{/_source.additionalInfo}}Anti-ASH1 | Histone-lysine N-methyltransferase ASH1
(Cat#: AS14 2816)
Description
- Immunogen: N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunolocalization (IL), Chromatin Immunoprecipitation (ChIP)
- Recommended dilutions: 1 : 500 (IL), 10ng/ul (ChIP)
- Expected | apparent MW: 236 | 300 kDa
- Confirmed reactivity: Drosophila melanogaster
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
- Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print].Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
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