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{{/_source.additionalInfo}}Anti-MIP1 | Aquaporin, glycerol transport activity
(Cat#: AS15 2826)
Description
- Immunogen: KLH-conjugated synthetic peptide, derived from Chlamydomonas reinhardtii MIP1, UniProt: Q5VLJ9
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunogold (IG), Western blot (WB)
- Recommended dilutions: 1 : 200 (IG), 1 : 10 000 (WB)
- Expected | apparent MW: 31.5 | 43 kDa
- Confirmed reactivity: Chlamydomonas reinhardtii (strains CC3395, and UVM4 from Neupert et al., 2009. (Generation of Chlamydomonas Strains that Efficiently Express Nuclear Transgenes.)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- MIP1 (Aquaporin, glycerol transport activity) is localized to the contractile vacuole, which in most freshwater flagellates is used to expel excess water.
- Komsic-Buchmann et al. (2014). The Contractile Vacuole as a Key Regulator of Cellular Water Flow in Chlamydomonas reinhardtii. Eukaryotic cell (13), Issue 11: 1421-30.
- As MIP1 is a membrane protein please use a high redox potential in your lysis buffer
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