Anti-NAD6 | NADH-ubiquinone oxidoreductase chain 6

(Cat#: AS15 2926)
AS15_2926
AS15_2926_1
Description
  • Immunogen: KLH-conjuagted peptide derived from Arabdiopsis thaliana sequence, UniProt: G1C2Y0, TAIR:ATMG00270
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Expected | apparent MW: 23.4 kDa (Arabidopsis thaliana), 25 kDa (Zea mays)
  • Confirmed reactivity: Arabidopsis thaliana
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • NAD6 (NADH-ubiquinone oxidoreductase chain 6) is involved in cellular respiration, oxidation-reduction process. Protein is a part of mitochondrial respiratory chain complex I and displays NADH dehydrogenase activity.
  • Wei et al. (2019). Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis. J Exp Bot. 2019 Aug 6. pii: erz357. doi: 10.1093/jxb/erz357.Colas des Francs-Small et al. (2018). Targeted cleavage of nad6 mRNA induced by a modified pentatricopeptide repeat protein in plant mitochondria. Commun Biol. 2018 Oct 11;1:166. doi: 10.1038/s42003-018-0166-8.
  • There is a cross-reaction with a thylakoid protein, therefore this antibody may not be usable for western blots of total leaf extracts

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