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{{/_source.additionalInfo}}Anti-RbcL II | Rubisco large subunit, form II
(Cat#: AS15 2955)
Description
- Immunogen: KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1:10 000 (IF), (WB)
- Expected | apparent MW: 51.7 kDa
- Confirmed reactivity: Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum
- Not reactive in: Burkholderi, Cyanobium sp.
- Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. Form II Rubisco is present in many photosynthetic bacteria and archaea and in some photosynthetic dinoflagellates. The large subunit (LSU) of form I Rubisco is encoded by the RbcL/cbbL gene, while form II LSU is encoded by cbbM.
- Cho et al. (2021). SxtA localizes to chloroplasts and changes to its 3'UTR may reduce toxin biosynthesis in non-toxic Alexandrium catenella (Group I). Harmful Algae, 2021,101972,ISSN 1568-9883, https://doi.org/10.1016/j.hal.2020.101972. ImmunolocalizationBausch et al. (2019). Combined effects of simulated acidification and hypoxia on the harmful dinoflagellate Amphidinium carterae. Marine Biology, June 2019, 166:80.Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
- Protein extraction from diatoms protocol can be found here.
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