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{{/_source.additionalInfo}}Anti-RBOHD | Respiratory burst oxidase homolog protein D
(Cat#: AS15 2962)
Description
- Immunogen: KLH-conjugated peptide chosen from Arabidopsis thaliana RBOHD sequence, UniProt:Q9FIJ0, TAIR: AT5G47910
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 104 kDa
- Confirmed reactivity: Arabidopsis thaliana, Solanum sp.
- Not reactive in: Marchantia polymorpha, Medicago sativa
- RBOHD (Respiratory burst oxidase homolog protein D) is a Calcium-dependent NADPH oxidase that generates superoxide. Is involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling.
- Goto et al. (2024). The leucine-rich repeat receptor kinase QSK1 regulates PRR-RBOHD complexes targeted by the bacterial effector HopF2Pto.Plant Cell. 2024 Oct 21:koae267.doi: 10.1093/plcell/koae267. Burian et al (2023) A prospective study of short-term apoplastic responses to ammonium treatment. J Plant Physiol. 2023 Jul;286:154008. doi: 10.1016/j.jplph.2023.154008.George J, Stegmann M, Monaghan J, Bailey-Serres J, Zipfel C. Arabidopsis translation initiation factor binding protein CBE1 negatively regulates accumulation of the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D. J Biol Chem. 2023 Jul 7:105018. doi: 10.1016/j.jbc.2023.105018.Qi et al. (2023) . Overexpression of an Antioxidant Enzyme APX1 in cpr5 Mutant Restores its Pleiotropic Growth Phenotype. Antioxidants. 2023; 12(2):301. https://doi.org/10.3390/antiox12020301.Wang et al. (2021) Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity. New Phytol. 2021 Dec 17. doi: 10.1111/nph.17922. Epub ahead of print. PMID: 34918346Lee et al. (2020). Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD. Nat Commun. 2020 Apr 15;11(1):1838. doi: 10.1038/s41467-020-15601-5.Jedelská et al. (2019). Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase. Biomolecules. 2019 Aug 21;9(9). pii: E393. doi: 10.3390/biom9090393.Otulak-Kozie? et al. (2019). The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions. Int. J. Mol. Sci. 2019, 20(11), 2741. (innunofluorescence)
- For solubilization we recommend 65°C for 5 min, Sample boiling is not recommended
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