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{{/_source.additionalInfo}}Anti-GFP | Green Fluorescence Protein (affinity purified)
(Cat#: AS15 2987)
Description
- Immunogen: Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Liquid
- Quantity: 100 µg at 1 mg/ml
- Storage: Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
- Confirmed reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
- GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
- Canal et al. (2024).Cytochrome c levels affect the TOR pathway to regulate growth and metabolism under energy-deficient conditions. New Phytol. 2024 Mar;241(5):2039-2058. Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelate et al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11(10):1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
- 10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
