Anti-RFP | Red Fluorescent Protein (clone RF5R)

(Cat#: AS15 3028)
Description
  • Immunogen: KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store at -20°C for up to 2 years.
  • Tested applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
  • Recommended dilutions: 1 : 1000-3000 (WB)
  • Confirmed reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.
  • Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.
  • Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

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